services

Custom enzyme engineering
for industry.

We design, evolve and produce enzymes tailored to your exact process — under strict NDA. From the first sequence design decision to kg-scale production, the same team throughout.

+30

industrial clients

6/8

top pharma trust us

21

funded R and D projects

100%

NDA by default
EvoEnzyme Laboratory 01
EVOSHUFFLER®
HTP Screening
Pilot 5-30L
line 1 — Industrial projects

01

Custom enzyme engineering for industry.

We work under strict NDA to design, evolve and produce enzymes tailored to your exact process. Every project is scoped individually with your team — no standard packages.

our platform

Three technologies.
One convergent platform.

We combine directed evolution, AI-guided computational design and ancestral sequence reconstruction — reaching a search space no single-method platform can cover.

EVOSHUFFLER® Directed Evolution

Explores where rational design cannot go

S. cerevisiae as evolution machine — random mutagenesis, in vivo DNA recombination, HTP screening under your selective pressure.

AI-guided Computational Design

Fewer rounds. Higher hit rate.

Structure-based AI predicts beneficial mutations at distal sites — reducing experimental search space before a single variant is screened.

Ancestral Sequence Reconstruction

Better starting point = better enzyme

Mesozoic-era fungal enzyme scaffolds inherently more thermostable, broadly promiscuous, more evolvable than modern sequences.

Entry points

Three ways
to work with us.

Every enzyme challenge is different. Tell us yours and we will propose the most effective approach.

Optimise your existing enzyme

Typical challenge

Active at pH 7, stable to 50 degrees C. Your process needs pH 4-5 and 70 degrees C — or higher selectivity on a specific substrate.

Also: solvent resistance · expression yield improvement · selectivity shift · reduced inhibition

Same function. Clean IP.

Typical challenge

A competitor patent blocks your process. You need equivalent performance with full freedom to operate — without licensing fees.

Also: supply independence · host compatibility · cost reduction · regulatory compliance

Build from scratch

Typical challenge

Novel reaction with no known enzymatic precedent. We identify, design and evolve the right biocatalyst for your transformation.

AI-guided discovery · ancestral resurrection · full directed evolution campaign

How a project unfolds

From briefing
to delivery.

A typical directed evolution project runs in four phases — with regular milestone reports and direct communication with our scientists throughout.

Phase 1

Define

Technical briefing, substrate analysis, starting enzyme selection, project scope.

Timeline and milestones agreed
Phase 2

Build and screen

Library generation, HTP screening, iterative evolution cycles under your selective pressure.

Regular milestone reports
Phase 3

Characterise

Full biochemical characterisation under your process conditions. Rigorous data package.

Data package delivered
Phase 4

Scale and deliver

Pilot (5-30L in-house) then industrial production supervised by our team. IP options discussed.

Timeline depends on scale
sectors

Nine sectors.
One evolved enzyme platform.

Pharmaceutical

Drug metabolites, API synthesis, HDM production

Fine chemistry

Chiral synthesis, green oxidations, cascade reactions

Bioremediation

Pollutant degradation, wastewater treatment

Renewable energy

Biofuel cells, H2 production, biomass conversion

Plastic degradation

PET depolymerisation, enzymatic recycling

Textile

Fibre processing, biofinishing, dyeing

Medical devices

Biosensors, diagnostics, therapeutic enzymes

Food and feed

Processing enzymes, flavour development

Synthetic biology

Custom enzymes for protocells, cell-free systems, minimal metabolism

Discuss your
enzyme challenge.

No standard packages. Every project starts with a conversation. We will respond within one working day with an initial assessment.

line 2 — Academic & Consortium R&D

02

The enzyme engineering partner
your consortium needs.

21 funded projects — EU Horizon, CDTI, NEOTEC, Comunidad de Madrid. We know how to deliver inside a public R and D consortium, from application to results.

EU Horizon

European R and D consortia

CDTI / NEOTEC

National R and D programmes

Comunidad de Madrid

Regional innovation funding

21 projects funded

Active track record

Looking for a technical partner?

Whether you are preparing a funding application or already have a project in motion and need enzyme engineering expertise — we are open to discussing how we can contribute.

  • EVOSHUFFLER® platform and enzyme library for consortium deliverables

  • Pilot production (5-30L in-house) for proof-of-concept milestones

  • IP management and co-ownership structures adapted to your programme

  • Co-authorship in peer-reviewed scientific publications

  • Expertise in EU Horizon, CDTI and Comunidad de Madrid regulations