We Are Evolvers

DIRECTED EVOLUTION: BRINGING THE CYCLE OF LIFE TO THE LAB BENCH

Directed evolution is an engineering strategy to customize enzymes for different purposes, ranging from industrial and environmental applications to drug discovery. Mimicking the processes of natural evolution in the laboratory, this strategy pioneered by Prof. Frances H. Arnold and for which she was awarded the Nobel Prize in Chemistry 2018, is considered the most revolutionary protein engineering method known to date.

First, genetic diversity is created by random mutagenesis and/or the recombination of parental genes. Once the mutant libraries are expressed in a suitable host organism, they can be screened  in high-throughput platforms to search for specific biochemical traits (e.g. to be active under extreme pHs or temperatures, to change substrate preferences, to be heterologously expressed, to tolerate the presence of strong inhibitors, etc.). In this way, the selective pressure is strictly controlled by the researcher until the desired properties are attained.

We can help you to develop your directed evolution platforms as well as to design enzymes for a variety of applications, from bioremediation to novel green processes.