Our Technology

Three technologies.
One platform.
Enzymes no one else can make.

We combine directed evolution, AI-guided computational design and ancestral sequence reconstruction in EVOSHUFFLER® — covering a search space no single-method platform can reach. From molecular design to industrial-scale production.

20+

peer-reviewed publications

4

IP-field patents

21

funded R&D projects

3

journal covers
Directed
Evolution
AI-guided
Design
Ancestral
Resurrection
EVOshuffler®
Enzymes no one else can make

“The convergence of directed evolution with computational design and ancestral resurrection gives us access to enzyme properties that evolution alone — even with millions of years — would not necessarily produce.

Miguel Alcalde

Co-founder & Scientific Advisor · Professor of Research, CSIC

One of the world’s leading researchers in directed evolution of enzymes — over 20 years pioneering the engineering of fungal oxidoreductases. Author of landmark publications in the field.

Professor of Research — CSIC Spain | Nature Chemical Biology 2025 | JACS · ACS Catalysis | 3 Journal Covers | SLAS EU 2023 keynote

The convergent platform

Why convergence
changes everything.

Each technology solves a different problem. Together they produce enzymes that wouldn’t be possible otherwise — and succeed on projects others have already tried and failed.

Directed Evolution

Saccharomyces cerevisiae as recombination platform

Finds unexpected variants through evolutionary pressure — beneficial mutations in unpredictable positions that no computational model would predict.

AI-guided Design

Accelerates convergence, reduces cost

Smarter libraries mean fewer iterations — faster delivery, lower experimental cost, higher hit rate per evolution round.

Ancestral Resurrection

Gives evolution a head start

Ancestral scaffolds are more evolvable — campaigns reach targets faster with properties modern sequences simply cannot match.

A hit rate and catalytic range that single-method platforms cannot match.
This is why 6 of the world’s top 8 pharma companies trust our enzymes — and why we take on projects others have already attempted and abandoned.

Full value chain

From molecular design
to industrial-scale production.

We cover every step — from the first computational design decisions to kg-scale production supervised by our team. No handoffs. The same team throughout.

Phase 1

Molecular design

Ancestral scaffolds + AI library design.

Ancestral + AI
Phase 2

Directed evolution

EVOshuffler® iterative cycles.

EVOshuffler®
Phase 3

Characterisation

Full biochemical characterisation.

In-house
Phase 4

Pilot production

5L & 30L in-house bioreactors.

In-house
Phase 5

Industrial scale

m³ – kg — partner network.

Supervised

Full value chain coverage — from the first sequence design decisions to industrial kg-scale production. No handoffs. No loss of control. The same team from molecule to manufacturing.

Scientific output

20+ publications.
3 journal covers.

Our technology is documented in the field’s most respected journals — not just internal validation.

JOURNAL COVERS
Journal Cover
Directed evolution of a highly selective fungal peroxygenase for the synthesis of indigo

Beltran-Nogal, A. et al. (2026).

Advanced Synthesis & Catalysis
doi.org/10.1021/acssuschemeng.6c02535

Journal Cover
Stable and promiscuous galactose oxidases engineered by directed evolution, atomistic design, and ancestral sequence reconstruction

Keser, M. et al. (2025).

ACS Synthetic Biology. 14, 1, 239–246. JOURNAL COVER.
https://doi.org/10.1021/acssynbio.4c00653.

Selected by ACS for a Collection celebrating Darwin Day 2025.
https://pubs.acs.org/page/vi/darwin-day-2025.

Journal Cover
Structural Insights and Reaction Profile of a New Unspecific Peroxygenase from Marasmius wettsteinii Produced in a Tandem-Yeast Expression System

Sánchez-Moreno, I. et al. (2024).

ACS Chemical biology, 19(10), 2240–2253. JOURNAL COVER.
https://doi.org/10.1021/acschembio.4c00504.

Journal Cover
Ancestral Resurrection and Directed Evolution of Fungal Mesozoic Laccases.

Gómez-Fernández, B.J. et al. (2020).

Applied and Environmental Microbiology 86 (14): e00778-20. JOURNAL COVER. https://aem.asm.org/content/86/14/e00778-20.abstract.

ALL PUBLICATIONS
Promiscuous fungal peroxygenase from Chaetomium globosum by directed evolution

Upp, D.M. et al. (2026).

Advanced Synthesis & Catalysis
doi.org/10.1016/j.isci.2026.117534

Structural features and reaction profile of an evolved unspecific peroxygenase from Candolleomyces aberdarensis

Menes-Rubio, A. et al. (2026).

Advanced Synthesis & Catalysis
doi.org/10.1002/adsc.70432

Directed evolution on the Aga2 subunit enhances laccase activity in the yeast surface display system

Teymennet-Ramírez, K. et al. (2026).

Biocatalysis and Biotransformation
doi.org/10.1080/10242422.2026.2638211

Directed evolution of LaccID for cell surface proximity labeling and electron microscopy

Lee, S-Y. et al. (2025).

Nature Chemical Biology
doi.org/10.1038/s41589-025-01973-6

Protein purification protocols for recombinant enzymes produced in Pichia pastoris

Keser, M. et al. (2025).

Methods in Molecular Biology. 2697: 657-667.
https://doi.org/10.1007/978-1-0716-4779-0_43.

Engineering the Tobacco Etch Virus protease towards a platform for traceless cleavage using distal site prediction and smart library design

Bemelmans, M. P. et al. (2025).

ACS Synthetic Biology. 14(9), 3721–3733.
https://doi.org/10.1021/acssynbio.5c00423.

Characterization of recombinant unspecific peroxygenase from Candolleomyces aberdarensis through crystallographic and substrate selectivity studies

Menes-Rubio, A. et al. (2025).

ChemCatChem. 17, e202402015.
https://doi.org/10.1002/cctc.202402015.

Engineering unspecific peroxygenases by structure-guided in vivo recombination of homologous protein blocks

Beltran-Nogal, A. et al. (2025).

Methods in Enzymology: Biocatalysis. 714.
https://doi.org/10.1016/bs.mie.2025.01.008.

Enzyme benchmarking with polyethylene furanoate soluble scaffolds for directed evolution of PEFases

Dolz, M. et al. (2024).

ACS Omega. 9, 45, 45633-45640.
https://doi.org/10.1021/acsomega.4c09053.

The colors of peroxygenase activity: Colorimetric high-throughput screening assays for directed evolution

Dolz, M. et al. (2023).

Methods in Enzymology: P450 enzymes. Elsevier. Vol 693: 73-109.
https://doi.org/10.1016/bs.mie.2023.09.006.

Engineering a highly regioselective fungal peroxygenase for the synthesis of hydroxy fatty acids.

Gomez de Santos, P. et al. (2022).

Angewandte Chemie International Edition. e202217372.
https://doi.org/10.1002/anie.202217372.

Surfing the wave of oxyfunctionalization chemistry by engineering fungal unspecific peroxygenases.

Beltran-Nogal, A. et al. (2022).

Current Opinion in Structural Biology 73: 102342.
https://doi.org/10.1016/j.sbi.2022.102342.

Directing the evolution of the fungal ligninolytic secretome.

Viña-Gonzalez, J. et al. (2021).

In: Protein Engineering: Tools and Applications. Wiley-VCH. 295-311.
https://doi.org/10.1002/9783527815128.ch12.

Directed evolution of the unspecific peroxygenase from Agrocybe aegerita in organic solvents.

Martin-Diaz, J. et al. (2021).

Biotechnology & Bioengineering 118: 3002-3014.
https://doi.org/10.1002/bit.27810

Recent developments in the use of peroxygenases – exploring their high potential in selective oxyfunctionalisations.

Hobisch, M. et al. (2021).

Biotechnology Advances 51: 107615. https://doi.org/10.1016/j.biotechadv.2020.107615

Engineering a Highly Thermostable High-Redox Potential Laccase.

Mateljak, I. et al. (2021).

ACS Sustainable Chem. Eng. 9, 29, 9632–9637. https://pubs.acs.org/doi/10.1021/acssuschemeng.1c00622.

Evolved peroxygenase-aryl alcohol oxidase fusions for self-sufficient oxyfunctionalization reactions.

Gómez-Fernández, B.J. et al. (2020).

ACS Catalysis 10: 13524-13534. https://pubs.acs.org/doi/10.1021/acscatal.0c03029

In vivo site-directed recombination (SDR): An efficient tool to reveal beneficial epistasis.

Viña-Gonzalez, J. et al. (2020).

In: Methods in Enzymology 643. Academic Press Elsevier Inc. Pages 1-12. https://doi.org/10.1016/bs.mie.2020.04.021.

Laccase engineering by directed and computational evolution.

Mateljak, I. et al. (2020).

In: Bioremediation and Waste valorization. Springer Nature Switzerland. 191-206. https://link.springer.com/chapter/10.1007/978-3-030-47906-0_8.

Benchmarking of laboratory evolved unspecific peroxygenases for the synthesis of human drug metabolites.

Gomez de Santos, P. et al. (2019).

Tetrahedron 75: 1827-1831. https://doi.org/10.1016/j.tet.2019.02.013.

theses
THESIS
Development of biotechnological platforms for the enzymatic degradation of polyesters

Student: Mikel Dolz
University: Universidad Autónoma de Madrid
Date: 03/07/2026

Want to apply this platform
to your enzyme challenge?

Our technology is available through directed evolution projects, screening kits and catalogue enzymes. Every engagement starts with a conversation.