NEW — Multi-Laccase Screening Kit

EVO LACkit

An exclusive collection of high-redox potential laccases

The first laccase screening kit on the market built from evolved white-rot fungal laccases. O₂ as the sole oxidant, water as the only by-product — ideal green biocatalysts for oxidation of phenols, polyphenols, aromatic amines and other electron-rich substrates.

16

evolved laccase
variants

96

reactions
per kit

O₂

sole
oxidant

3

redox
mediators
What is in the kit
16 evolved UPO variants (lyophilized)

96-deep well plate format — 6X each enzyme, screening 6 test compounds per variant

Reaction buffer vial

Optimised for laccase activity across different substrates

3 redox mediators

HBT, TEMPO and ABTS — for laccase mediator system (LMS) reactions

96 reactions per kit. All variants recombinantly produced in yeast (S. cerevisiae). Custom formats available on request.

The laccase advantage

Why laccases over conventional chemical oxidants?

Laccases are multi-copper oxidoreductases that use only oxygen from the air, releasing water as the only by-product — a green alternative to harsher chemical oxidation routes.

Conventional chemical oxidants
Stoichiometric reagents
Consumed in excess, generating chemical waste streams.
Harsh reaction conditions
Often require extreme pH, temperature or metal catalysts.
Poor selectivity
Over-oxidation and unwanted side products.
Limited substrate range
Bulky or high-redox-potential compounds often unreactive.
EVO LACkit laccases
O₂ as sole oxidant
From air, releasing only water as by-product.
Secreted, soluble enzymes
Recombinantly produced in yeast — easy to handle.
High-redox potential variants
Evolved to oxidize a broader range of molecules.
Extendable with mediators
Laccase mediator system reaches bulky, high-redox substrates.
Catalytic repertoire

Broad oxidation power
on electron-rich substrates.

The laccases in EVO LACkit oxidize phenols, polyphenols, aromatic amines and other electron-rich compounds — and, combined with redox mediators, reach substrates that other laccases can’t touch.

Direct oxidation

Classic laccase chemistry — oxidation of phenols, polyphenols and aromatic amines using only O₂, with water as the sole by-product.

e.g. phenolics · polyphenols · aromatic amines

Laccase Mediator System (LMS)

For bulky substrates (e.g. polymers) or compounds with high redox potential, diffusible mediators extend the laccase’s oxidative reach.

HBT · TEMPO · ABTS — three distinct oxidative mechanisms included

HBT mediator

Hydrogen transfer mechanism

TEMPO mediator

Ionic mechanism

ABTS mediator

Electron transfer mechanism

High-redox variants

Engineered for challenging reaction conditions

Laccases are considered ideal green biocatalysts, with applications spanning bioremediation, organic synthesis, biofuel cells, biosensors, textiles, pulp and paper, and food and cosmetics.

Case studies

Published applications.
Real results.

Almost two decades of directed evolution on fungal laccases, documented across peer-reviewed publications from our founding team.

Solvent tolerance
Chemistry & Biology 2007
In vitro evolution of a fungal laccase in high concentrations of organic cosolvents

Directed evolution campaign engineering laccase activity and stability under organic cosolvent conditions relevant to industrial biocatalysis

Zumárraga et al. · Chemistry & Biology 14(9), 1052–1064 · 2007

High-redox potential
Chemistry & Biology 2010
Laboratory evolution of high-redox potential laccases

Engineering campaign that raised laccase redox potential — foundational work behind the high-redox variants in EVO LACkit

Maté et al. · Chemistry & Biology 17(9), 1030–1041 · 2010

Redox & mediators
ACS Catalysis 2019
Increasing Redox Potential, Redox Mediator Activity, and Stability in a Fungal Laccase by Computer-Guided Mutagenesis and Directed Evolution

Computer-guided design combined with directed evolution improved both redox potential and mediator (LMS) activity — the same principle behind EVO LACkit’s mediator system

Mateljak et al. · ACS Catalysis 9(5), 4561–4572 · 2019

Your substrate
Exploratory assay

Have a specific substrate you’d like to test?

Send us your compound — we run the exploratory assay at our facilities and deliver a full report with the best laccase candidates and optimization recommendations.

From kit to scale

The kit is the first step.

Step 1

Screen with the kit

Test 16 laccase variants on your substrate, with or without mediators

You are here
Step 2

Identify your hit

Best variant on your substrate — confirmed by activity data

Screening output
Step 3

Discuss Scale up

Contact us about larger volumes for your application

Scale-up
Step 4

Evolve further

Custom directed evolution campaign to optimize further

Optional

All EVO LACkit variants are recombinantly produced in Saccharomyces cerevisiae. Once a positive hit is identified, we can work with you to supply the mutant of choice in larger amounts.

Additional services

Beyond the kit.
Full support.

Larger volumes on request

Contact us to discuss larger quantities of desired laccase variant.

Tailored EVO LACkit

Custom kit formats for specific testing scenarios — adapted for multiple chemical entity screening, with mediator combinations tuned to your compound class.

In-house testing and analysis

We run the exploratory assay at our facilities for a specific biotransformation or challenge. After careful analysis, a detailed report identifies the best candidate enzymes and optimization recommendations.

Laccase customization via Directed Evolution

Once the best candidate is chosen, we offer further development through ad-hoc directed evolution campaigns — including HTP assay design, library creation via EVOSHUFFLER® and scale-up production.

Green oxidation for industrial applications

Applications spanning bioremediation, biofuel cells and biosensors, textiles, pulp and paper, and food and cosmetics — wherever a mild, O₂-driven oxidation step is needed.

Not sure where to start?

Our scientists will analyse your specific needs and recommend the best approach — whether it’s the kit, an exploratory assay or a full custom project.

Ready to try EVO LACkit
on your substrate?

Request information, order a kit, or send us your substrate for an exploratory assay — our scientists will get back to you within 24–48 hours.