Evolved Enzyme Library

Six enzyme families.
Evolved in our lab.
Available today.

A curated portfolio of unique biocatalysts — not sourced from databases, but engineered through directed evolution, AI-guided design and ancestral reconstruction. Backed by 20+ peer-reviewed publications.

6

enzyme families

100mg→kg

available scale

20+

publications

S.cer

yeast expression
Evolved
Enzyme
Library
UPOs6+ pubs
AAOs3+ pubs
Laccases8+ pubs
UricasesMedical
CutinasesACS Omega
VPs4+ pubs
What makes this library different

These enzymes were
engineered, not found.

Evolved, not isolated

Every variant has been engineered through iterative directed evolution — selected for superior activity, stability or selectivity under real industrial conditions. Not wild-type isolates from public databases.

Backed by 20+ publications

Performance documented in JACS, Angewandte Chemie, ACS Catalysis and ACS Synthetic Biology — not just internal claims. Peer-reviewed science behind every family.

Production-ready

Produced in our yeast expression system with established downstream protocols. Available from 100 mg to kg — CoA and full technical documentation provided on request.

UPOS · 6+ PUBLICATIONS

Fungal Unspecific Peroxygenases

Selective C-H oxyfunctionalization using H₂O₂ — the evolved alternative to P450 monooxygenases.

KEY ADVANTAGE

Emulates P450 chemistry without NADPH or regeneration systems — simpler, more robust and fully scalable.

APPLICATIONS

Human drug metabolites
Late-stage functionalization
C-H oxyfunctionalization Fine chemistry

JACS · ACS Chemical Biology · ACS Catalysis · Applied & Environmental Microbiology

Also available as EVO UPOkit

LACCASES · 8+ PUBLICATIONS

High-Redox Potential Fungal Laccases

Green oxidation using O₂ from air — water as sole by-product. Evolved for superior redox potential.

KEY ADVANTAGE

High-redox potential variants oxidise substrates inaccessible to wild-type laccases — with tolerance to extreme pH, temperature and organic solvents.

APPLICATIONS

Bioremediation Organic synthesis Textile Biosensors Biofuel cells

ACS Catalysis · ACS Synth. Biology · Chemistry & Biology · ACS Sustain. Chem.

Also available as EVO LACkit

VPS · 4+ PUBLICATIONS

Versatile Peroxidases

Multiple catalytic sites — high-redox potential with broad substrate scope and exceptional oxidative stability.

KEY ADVANTAGE

Combines MnP and LiP catalytic versatility in one enzyme — with evolved thermostability, extreme pH resistance and oxidative inactivation tolerance.

APPLICATIONS

Bioremediation Food industry
Organic synthesis Biosensors

Biochem. J. · ACS Catalysis · Catalysis Science & Technology

Request technical documentation

AAOS · 3+ PUBLICATIONS

Aryl Alcohol Oxidases

High enantioselectivity for chiral alcohol resolution — in-situ H₂O₂ generation for cascade reactions.

KEY ADVANTAGE

Evolved for high enantioselectivity on primary and secondary aromatic substrates — enables chiral alcohol resolution and self-sufficient cascade systems with UPOs.

APPLICATIONS

Chiral synthesis Cascade reactions
Green chemistry Fine chemistry

Adv. Synth. & Catal. · Biotechnology & Bioengineering · Applied Env. Microbiology

Request technical documentation

CUTINASES · ACS OMEGA 2024

Evolved Cutinases

Ester bond hydrolysis for plastic degradation — engineered for thermostability and activity on synthetic polyesters.

KEY ADVANTAGE

Evolved for high activity on PET, PEF, PBS and PBAT — enabling depolymerisation to pure monomers under mild conditions (pH 8, no extreme temperatures).

APPLICATIONS

PET depolymerisation Plastic recycling Circular economy Biodegradability

Dolz et al., ACS Omega 9(45), 45633–45640 (2024) · further data available on request

Also available as EVO CUTinase Cocktail

URICASES · MEDICAL

Evolved Uricases

Uric acid oxidation for medical and diagnostic applications — evolved for physiological stability and activity.

KEY ADVANTAGE

Evolved for activity and stability under physiological conditions — relevant for gout treatment research, uric acid biosensors and diagnostic quantification assays.

APPLICATIONS

Medical devices Diagnostics
Therapeutic enzymes Biosensors

Engineering data and performance characterisation available on request

Request technical documentation

Enzyme combinations

The most powerful results
come from combining families.

Our evolved variants are designed to work together. Several enzyme families from our library can be combined in cascade or self-sufficient systems for reactions not achievable with a single biocatalyst.

AAOs+UPOs

Self-sufficient peroxygenase cascade

AAOs generate H₂O₂ in situ from O₂ — feeding it directly to UPOs for C-H oxyfunctionalization. No external oxidant required. A fully self-sufficient, green oxidation system.

⚡
Published in ACS Catalysis — selective HDM synthesis without H₂O₂ addition

Laccases+VPs

Oxidative ligninolytic system

Laccases and versatile peroxidases work synergistically — covering different substrate classes and redox potentials for more complete ligninolytic transformation.

🌱
Broad-spectrum activity — bioremediation and biorefineries

UPOs+Laccases

Dual oxidase for fine chemistry

UPOs for selective C-H functionalization combined with laccases for downstream oxidative transformations — enabling multi-step biocatalytic sequences in a single pot.

🔗
One-pot biocatalytic pharmaceutical synthesis

Uricases+Peroxidases

Uric acid detection cascade

Uricases generate H₂O₂ from uric acid — which peroxidases use for colorimetric or electrochemical detection. A complete enzymatic cascade for biosensor development.

📈
Diagnostic biosensor — uric acid quantification
Availability & ordering

100 mg to kg.
Tell us what you need.

No catalogue to browse — no prices to compare. Just tell us what you need and we’ll confirm availability, timeline and the right format for your application.

Available scale

100 mg

g scale

kg

Research
Assay development
Pilot
Process development
Industrial
Full production

how it works

1 Tell us what you need

Enzyme family, quantity, purity and any formulation requirements.

2 We confirm in one working day

Availability, production timeline and pricing — no standard catalogue delays.

3 Delivered with full documentation

CoA, activity data, storage guide and ongoing technical support.

4 Need it to perform better?

Tailored directed evolution campaign to push activity, stability or selectivity further.

Part of the EvoEnzyme ecosystem

The library is one entry point.
There are others.

Not sure which enzyme to start with?

Try one of our EVO Screening Kits — evolved enzyme collections ready to screen in your lab today. UPOkit, LACkit or CUTinase Cocktail.

Need a custom enzyme for your process?

Our directed evolution service engineers enzymes to your exact specifications — thermostability, selectivity, expression system and scale.

Looking for a research partner?

We collaborate with academic groups and industrial consortia through funded R&D programmes — bringing our library and EVOSHUFFLER® to your project.

Looking for a specific enzyme
not listed here?

Our portfolio is continuously expanding. Tell us what you need and we’ll let you know if we have it — or if we can engineer it.