Directed enzyme evolution

We evolve enzymes so your chemistry
works better.

Custom biocatalysts for the pharmaceutical, chemical and environmental industries — faster, greener and tailored to your process. From molecular design to industrial-scale production.

Talk to our team

See our products

Trusted by

Repsol · Corteva · Servier · +30 clients

Merging

Directed Evolution · AI Design · Ancestral Resurrection

21

research grants

EU, CDTI, Spanish Ministry & Comunidad de Madrid

20+

publications

in JACS, Angewandte Chemie & ACS Synth Bio

6 of top 8

pharma

Global pharma companies trust our enzymes

4

IP-field patents

Proprietary innovations in enzyme engineering, directed evolution & biocatalysis platforms

Industries we serve

Pharmaceutical Fine chemistry Bioremediation Renewable energy Medical devices Food & feed Textiles Plastic recycling Synthetic biology

How it works

Define your challenge

Select & evolve

Screen & validate

Deliver your enzyme

who we are

EvoEnzyme designs customized biocatalysts
through directed evolution.

Founded by experienced staff scientists at the CSIC (Spanish National Research Council) with a strong background in applied biocatalysis, we provide enzyme-based solutions for the pharmaceutical, chemical and environmental industries.

From bioremediation to drug discovery — our cutting-edge technology connects industrial biotechnology with green chemistry.

Contributing to the UN Sustainable Development Goals

Products and services

Find the right solution
for your enzyme challenge

From fully custom directed evolution projects to ready-to-use screening kits and catalogue enzymes — tailored to your timeline, application and scale.

Not sure which solution fits your needs?

Tell us your challenge and our scientists will recommend the best approach — no commitment required.

Talk to our team

RESEARCH SERVICES

Directed evolution projects

We engineer enzymes to meet your exact technical requirements — activity, selectivity, stability, expression. Each project is scoped individually with your team.

PROJECT WORKFLOW

1

Define the challenge
2
Build & screen
3
Characterise & validate
4
Scale-up

TECHNICAL CAPABILITIES

Directed evolution + AI-guided design
Ancestral sequence reconstruction
Expression in yeast (S. cerevisiae, P. pastoris) & Bacteria (E. coli)
Scale-up from mg to kg

Learn more about our technology

SCREENING KITS

EVO enzyme kits

Ready-to-use kits containing our best evolved enzymes — designed for rapid activity screening in academic and industrial R&D settings.

AVAILABLE KITS

EVO UPOkit 3.0
Evolved peroxygenases for selective C-H oxyfunctionalization and human drug metabolite synthesis.

EVO LACkit
High-redox potential laccase collection for oxidation reactions using O₂.

EVO CUTinase Enzyme Cocktail
Evolved cutinases for synthetic polyester degradation screening.

Step-by-step protocol & positive controls
Scientific support from our team
Scale-up & customization on request

Request information

ENZYME CATALOGUE

Evolved enzyme library

A curated portfolio of evolved enzymes produced in yeast, available for industrial and research applications across multiple sectors.

ENZYME PORTFOLIO

Fungal UPOs — Selective C-H oxyfunctionalization, pharma & fine chemistry

Laccases — High-redox potential, oxidation, textile, biosensors

Versatile peroxidases (VPs) — Thermostable, extreme pH tolerance

Aryl alcohol oxidases (AAOs) — High enantioselectivity, chiral alcohol resolution

Cutinases — PET & synthetic polymer degradation

Uricases — Uric acid oxidation, medical & diagnostic applications

Produced by yeast expression system
Full technical documentation on request
Custom quantities available

Request information

Why EvoEnzyme

What sets
us apart.

We are the first Spanish company specialised in directed enzyme evolution — built on 20+ years of fundamental research at the CSIC.

We are scientists

Founded by experienced staff scientists at the CSIC with a strong background in applied biocatalysis. We understand your scientific and industrial needs from the inside.

Pioneering technologies

EVOSHUFFLER® directed evolution, AI-guided computational design and ancestral sequence reconstruction — converging three platforms into one.

We are flexible

We adapt our directed evolution protocols to your specific problem — obtaining optimal results in an affordable and scientifically rigorous manner.

We care for you

Customer relations are our top priority. We guarantee continuous communication and feedback with your laboratory throughout every project.

We are sustainable

We connect industrial biotechnology with green chemistry — contributing to the UN SDGs across sectors from renewable energy to circular economy.

Full value chain

From the first sequence design decision to kg-scale industrial production. No handoffs. The same team from molecule to manufacturing.

Explore our technology

A Trusted Company

6 of the world’s top 8 pharma companies
trust our enzymes

Dozens of companies and institutions already count on our evolved biocatalysts — from global pharma to industrial chemistry and academic research.

Presence

Less More

Spain

Germany

United States

France

Netherlands

Other markets: Switzerland, Sweden, China, Belgium, United Kingdom, Brazil, Denmark, India, Mexico, Italy, South Africa

testimonials

What our partners say
about us.

“Amazing relationship with EvoEnzyme since day one of our collaboration. A very professional enthusiastic, creative, and committed team that offers multiple technical possibilities to respond to our needs.”

Mar González Barroso

Technical Advisor in Biotechnology – Repsol

“The EVO UPOKit has been a pleasure to use in my screening.”

Matt Chase

Principal Investigator, Integrated Discovery & Bioprocess, Crop Health R&D – CORTEVA Agriscience

“We have chosen EvoEnzyme as our partner for the development of high-throughput screening assays and computational evolution related to the development of evolved variants of our Numacut TEV protease. We are very pleased with the implementation of the task and the specific project results. Thanks to the improved variants, we have completed our production platform Numaswitch for the production of peptides and proteins. We can unreservedly recommend EvoEnzyme and thank them for the excellent work and the scientific exchange at highest level.”

Florian Tieves

Head of Innovation Department – NUMAFERM

“I don’t believe in coincidences but… some time ago I received a request for a survey about biocatalysts and because that I knew a group of very professional scientists but specially very nice people working in a growing company called EvoEnzyme. After that we start a very interesting and productive collaboration that we hope will lead us to a good partnership.”

Gastón Ariel Estruch

Technical Director at GENTEC Pharmaceutical Group – Pharmanoid Site

“We have collaborated with EvoEnzyme for the last two years using their enzymes and technical knowledge and we couldn’t be more satisfied. It is very easy to work in technology with collaborators who are so prepared and close.”