Our departure point
Enzyme benchmarking and discovery
Thorough the biochemical characterizations we carry out, insight can be gained into the performance of your enzymes. As such, we can provide you with data and information regarding:
Enzyme activity
Selectivity
Substrate scope
Thermostability
pH profile
Organic solvent tolerance
and much more. In this way, we will be in a position to select the best candidate for your directed evolution campaign.
Moreover, if you still don´t have a suitable starting point for protein engineering, we can find candidate enzymes that might be well-suited to your specific task. This can be achieved by combining computational mining and ancestral reconstruction, or simply by searching candidates from our exclusive proprietary mutant libraries.
Taking directed evolution to the next level
Customised strategies for enzyme engineering
We apply modern directed evolution methods, together with cutting-edge computational approaches that can be tailored to your specific needs, addressing everything from mutant library construction and exploration, to ab initio enzyme design.
High-throughput screening assays
We can set-up very sensitive and reliable screening assays that are designed to identify improvements in different biochemical traits.
Mutant library creation – DNA recombination is our forte
In conjunction with random mutation, DNA recombination represents the main driver in natural evolution. At EvoEnzyme, we leverage this potent phenomenon and take it to the next level. Based on Saccharomyces cerevisiae, our proprietary technological platform EVOSHUFFLER® allows beneficial mutations to undergo recombination at the same time as detrimental mutations discarded in the directed evolution pipeline, all in a rapid and very precise manner.
In silico DNA recombination for the design of chimeric enzymes
Chimeric enzymes can be generated by swapping sequence blocks from different parental variants with a view to enhancing properties like stability, activity and expression. To design chimeric enzymes, EvoEnzyme employs computational SCHEMA-RASPP homologous recombination in conjunction with in vivo DNA shuffling. In this way, full repertoires of functional chimeras can be generated while introducing significant sequence diversity.
Ancestral sequence reconstruction
If the specific enzyme you desire cannot be found in natural sources or there is no freedom-to-operate, at EvoEnzyme we can perform ancestral sequence reconstruction through the phylogenetic inference of ancestral nodes and expression in modern hosts. Based on your query, we can generate novel ancestral enzymes with new sequences (thereby ensuring freedom-to-operate), which may then be used in future laboratory evolution campaigns.
Artificial intelligence-guided directed evolution
Within our portfolio of engineering methods, we are recurrently employing computational algorithms to approach the evolved enzymes to the global optima. This ranges from a priori and a posteriori simulations of ligand diffusion, to one-shot computational mutagenesis and the application of our own ML/DL (Machine Learning/Deep Learning) protocols within our directed evolution workflows.
Enzyme production
On demand scaling and downstream processing
At our bioreactor facilities, we can scale up your enzyme production from the mg to the g/Kg scale. We can produce your enzyme in three host organisms (Escherichia Coli, Saccharomyces cerevisiae and Pichia pastoris) depending on your needs, performing additional downstream and purification procedures upon request.