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Experts in Ligninolytic Enzymes
Experts in Ligninolytic Enzymes
Searching for new opportunities
Searching for new opportunities
Evoling to your needs
Evoling to your needs
“All terrain” enzymes
“All terrain” enzymes

WHAT WE DO

Directed enzyme evolution is our speciality: we developed directed evolution strategies to resolve your specific problem. Want to learn more about directed evolution? Click here: https://evoenzyme.com/technology.

Besides, EvoEnzyme commercializes evolved ligninolytic enzymes and mutant libraries of fungal peroxygenases, high-redox potential laccases, versatile peroxidases and aryl alcohol oxidases for pharmaceutical, chemical, and environmental applications.

Latest Publications
Engineering a Highly Thermostable High-Redox Potential Lacasse

Mateljak I, and Alcalde M (2021)

Engineering a Highly Thermostable High-Redox Potential Lacasse. 

ACS Sustainable Chem. Eng. 2021, 9, 29, 9632–9637
https://pubs.acs.org/doi/1 0.1021/acssynbio.8b00509.

Functional Expression of Two Unusual Acidic Peroxygenases from Candolleomyces aberdarensis in Yeasts by Adopting Evolved Secretion Mutations

Gómez de Santos, P; Hoang, MD; Kiebist, J; Kellner, H; Ullrich, R; Scheibner, K; Hofrichter, M; Liers, C and Alcalde, M (2021) 

Functional Expression of Two Unusual Acidic Peroxygenases from Candolleomyces aberdarensis in Yeasts by Adopting Evolved Secretion Mutations.
Applied and Environmental Microbiology 87 (19): e00878-21 https://pubs.acs.org/doi/10.1 021/acssynbio.8b00509.

Gómez de Santos, P., Lázaro, S., Viña-González, J., Hoang, M.D., Glieder, A., Hollmann, F. and Alcalde, M. (2020).

Evolved peroxygenase-aryl alcohol oxidase fusions for self-sufficient oxyfunctionalization reactions.
Ancestral Resurrection

Gómez-Fernández, B.J., Risso, V.A., Rueda, A., Sánchez-Ruiz, J.M. and Alcalde, M.(2020).

Ancestral Resurrection and Directed Evolution of Fungal Mesozoic Laccases.

Applied and Environmental Microbiology 86 (14): e00778-20. JOURNAL COVER.
https://pubs.acs.org/doi/10.1021/acssynbio.8b00509.

Methods in Enzymology

Viña-Gonzalez, J. And Alcalde, M. (2020).

In vivo site-directed recombination (SDR): An efficient tool to reveal beneficial epistasis. In: Methods in Enzymology 643.

Tawfik, D. Ed. Academic Press Elsevier Inc, Cambridge, MA, EEUU. Pages 1-12. ISBN 978-0-12-821149-6.
https://pubs.acs.org/doi/10.1021/acssynbio.8b00509.

Mateljak, I., Gomez-Fernandez, B. and Alcalde, M. (2020).

Laccase engineering by directed and computational evolution. In: Laccase in bioremediation of pollutants and xenobiotics

Bioremediation and Waste valorization. Schlosser, D., Ed. Springer Nature Switzerland. Pages: 191-206. ISBN: 978-3-030-47906-0
https://pubs.acs.org/doi/10.1021/acssynbio.8b00509.

Hobisch, M., Holtmann, D., Gomez de Santos, P., Alcalde, M., Hollmann, F. and Kara, S. (2020).
Recent developments in the use of peroxygenases – exploring their high potential in selective oxyfunctionalisations.

Biotechnology Advances. In press.
https://doi.org/10.1016/j.tet.2019.02.013

Gomez de Santos, P., Cervantes, F.V., Tieves, F., Plou, F.J., Hollmann, F. and Alcalde, M. (2019).
Benchmarking of laboratory evolved unspecific peroxygenases for the synthesis of human drug metabolites.
*For a full list of publications, see www.miguelalcaldelab.eu