Experts in Ligninolytic Enzymes

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Evolving to your needs

“All terrain” enzymes

What We Do

EvoEnzyme commercializes evolved ligninolytic enzymes and mutant libraries of fungal peroxygenases, high-redox potential laccases, versatile peroxidases and aryl alcohol oxidases for pharma, chemical, and environmental applications.

Directed enzyme evolution is our speciality: we developed directed evolution projects to solve your specific problem. Want to learn more about directed evolution? Click here: (


Mateljak, I., Rice, A., Yang, K., Tron, T. and Alcalde, M. (2019).

The generation of thermostable fungal laccase chimeras by SCHEMA-RASPP structure-guided recombination in vivo.

ACS Synthetic Biology 8: 833-843. JOURNAL COVER. (

Gomez de Santos, P., Cervantes, F.V., Tieves, F., Plou, F.J., Hollmann, F. and Alcalde, M. (2019).

Benchmarking of laboratory evolved unspecific peroxygenases for the synthesis of human drug metabolites.

Tetrahedron 75: 1827-1831. (


Structure-guided evolution of aryl alcohol oxidase from Pleurotus eryingii for the selective oxidation of secondary benzyl alcohols.

Advanced Synthesis and Catalysis. 361: 2514-2525. (

Mateljak, I., Monza, E., Lucas, M.F., Guallar, V., Aleksejeva, O., Ludwig, R., Leech, D., Shleev, S. and Alcalde, M. (2019).

Increasing redox potential, redox mediator activity and stability in a fungal laccase by computer-guided mutagenesis and directed evolution.

ACS Catalysis 9: 4561-4572. (

*Full list of publications at