Experts in Ligninolytic Enzymes

Searching for new opportunities

Evolving to your needs

“All terrain” enzymes

What We Do

Directed enzyme evolution is our speciality: we developed directed evolution strategies to resolve your specific problem. Want to learn more about directed evolution? Click here: (

Besides, EvoEnzyme commercializes evolved ligninolytic enzymes and mutant libraries of fungal peroxygenases, high-redox potential laccases, versatile peroxidases and aryl alcohol oxidases for pharmaceutical, chemical, and environmental applications.



Gómez de Santos, P., Lázaro, S., Viña-González, J., Hoang, M.D., Glieder, A., Hollmann, F. and Alcalde, M. (2020).

Evolved peroxygenase-aryl alcohol oxidase fusions for self-sufficient oxyfunctionalization reactions. 

ACS Catalysis 10: 13524-13534. (

Gómez-Fernández, B.J., Risso, V.A., Rueda, A., Sánchez-Ruiz, J.M. and Alcalde, M.(2020).

Ancestral Resurrection and Directed Evolution of Fungal Mesozoic Laccases.

Applied and Environmental Microbiology 86 (14): e00778-20. JOURNAL COVER. (

Viña-Gonzalez, J. And Alcalde, M. (2020).

In vivo site-directed recombination (SDR): An efficient tool to reveal beneficial epistasis. In: Methods in Enzymology 643.

Tawfik, D. Ed. Academic Press Elsevier Inc, Cambridge, MA, EEUU. Pages 1-12. ISBN 978-0-12-821149-6.(

Mateljak, I., Gomez-Fernandez, B. and Alcalde, M. (2020).

Laccase engineering by directed and computational evolution. In: Laccase in bioremediation of pollutants and xenobiotics.

Bioremediation and Waste valorization. Schlosser, D., Ed. Springer Nature Switzerland. Pages: 191-206. ISBN: 978-3-030-47906-0


Hobisch, M., Holtmann, D., Gomez de Santos, P., Alcalde, M., Hollmann, F. and Kara, S. (2020).

Recent developments in the use of peroxygenases – exploring their high potential in selective oxyfunctionalisations.

Biotechnology Advances. In press. (

Gomez de Santos, P., Cervantes, F.V., Tieves, F., Plou, F.J., Hollmann, F. and Alcalde, M. (2019).

Benchmarking of laboratory evolved unspecific peroxygenases for the synthesis of human drug metabolites.

Tetrahedron 75: 1827-1831. (

*For a full list of publications, see