What We Do

Directed enzyme evolution is our speciality: we developed directed evolution strategies to resolve your specific problem. Want to learn more about directed evolution? Click here: (https://evoenzyme.com/technology/).

Besides, EvoEnzyme commercializes evolved ligninolytic enzymes and mutant libraries of fungal peroxygenases, high-redox potential laccases, versatile peroxidases and aryl alcohol oxidases for pharmaceutical, chemical, and environmental applications.


Gómez de Santos, P., Lázaro, S., Viña-González, J., Hoang, M.D., Glieder, A., Hollmann, F. and Alcalde, M. (2020).

Evolved peroxygenase-aryl alcohol oxidase fusions for self-sufficient oxyfunctionalization reactions. 

ACS Catalysis 10: 13524-13534.(https://pubs.acs.org/doi/ 10.1021/acssynbio.8b00509).

Ancestral Resurrection

Gómez-Fernández, B.J., Risso, V.A., Rueda, A., Sánchez-Ruiz, J.M. and Alcalde, M.(2020).

Ancestral Resurrection and Directed Evolution of Fungal Mesozoic Laccases.

Applied and Environmental Microbiology 86 (14): e00778-20. JOURNAL COVER.(https://pubs.acs.org/doi/ 10.1021/acssynbio.8b00509).

Methods in Enzymology

Viña-Gonzalez, J. And Alcalde, M. (2020).

In vivo site-directed recombination (SDR): An efficient tool to reveal beneficial epistasis. In: Methods in Enzymology 643.

Tawfik, D. Ed. Academic Press Elsevier Inc, Cambridge, MA, EEUU. Pages 1-12. ISBN 978-0-12-821149-6.(https://pubs.acs.org/doi/10. 1021/acssynbio.8b00509).

Mateljak, I., Gomez-Fernandez, B. and Alcalde, M. (2020).

Laccase engineering by directed and computational evolution. In: Laccase in bioremediation of pollutants and xenobiotics.

Bioremediation and Waste valorization. Schlosser, D., Ed. Springer Nature Switzerland. Pages: 191-206. ISBN: 978-3-030-47906-0

(https://pubs.acs.org/doi /10.1021/acssynbio.8b00509).

Hobisch, M., Holtmann, D., Gomez de Santos, P., Alcalde, M., Hollmann, F. and Kara, S. (2020).

Recent developments in the use of peroxygenases – exploring their high potential in selective oxyfunctionalisations.

Biotechnology Advances. In press. (https://doi.org/10.1016/ j.tet.2019.02.013)

Gomez de Santos, P., Cervantes, F.V., Tieves, F., Plou, F.J., Hollmann, F. and Alcalde, M. (2019).

Benchmarking of laboratory evolved unspecific peroxygenases for the synthesis of human drug metabolites.

*For a full list of publications, see www.miguelalcaldelab.eu