Is the first screening kit in the market that comprises a selection of evolved fungal unspecific peroxygenases (UPOs), and it has been designed to achieve scalable production of chemicals and human drug metabolites. Coming from different fungal species (short and long-type UPO families), our exclusive collection of evolved UPOs can perform an extensive range of selective oxyfunctionalizations on pharmacological compounds, emulating the role of human liver P450 monoxygenases. Unlike P450s, UPOs simply use H2O2 as an oxidant, making laboratory manipulations easier and more robust. The catalytic repertoire of the selected UPOs in EVOkit are relevant to both the pharmaceutical and chemical sector, and its portfolio includes alkyl and aromatic hydroxylations, aliphatic and aromatic epoxidations, O-dealkylations, N-dealkylations (including ester and ether cleavage), N-oxidations, S-oxidations and brominations.
EvoEnzyme’s fungal peroxygenase kit is presented in glass vials designed to carry out efficient and directed screening, following a step-by-step protocol. EVOkit contains 12 evolved UPO variants carefully selected for their catalytic properties, encompassing a wide array of biotransformations and selectivity. To make the assay easier, EVOkit contains three accessory vials with reaction buffer, hydrogen peroxide and a UPO substrate to be used as a positive control. We also offer constant support to our customers to assist them with any specific reactions they wish to carry out with the EVOkit
EVOkit Case Study
Larger quantities of our evolved UPO enzymes are available upon request. A Pichia pastoris expression system has been adapted, and production protocols established, for the bioreactor secretion of soluble, very stable, extracellular peroxygenases. EvoEnzyme also has in place downstream protocols and handling instructions for the secure supply of any desired products.
EvoEnzyme can prepare kits with alternative formats adjusted to specific testing scenarios. In this sense, we can adapt EVOkit to the screening of multiple chemical entities. Also the Company is developing non-standard versions of the kit with immobilized enymes.